Journal: Cell proliferation

Article Title: Ellagic Acid-Loaded sEVs Encapsulated in GelMA Hydrogel Accelerate Diabetic Wound Healing by Activating EGFR on Skin Repair Cells.

doi: 10.1111/cpr.70064

Figure Lengend Snippet: FIGURE 4 | EGFR was identified as a target protein of EA. (A) Target proteins of EA were predicted by SwissTargetPrediction, Super Pred, SEA and Pharmmapper database and three proteins were screened out. (B-D) GO enrichment analysis results were classified according to BP, CC and MF. The top 10 GO term items with the minimum p value and the most significant enrichment in each GO category were selected. (E) KEGG pathway enrichment analysis of EA. The top 10 KEGG pathways with the smallest p value were selected. (F) Western blot analysis showing the expression levels of EGFR, INSR and C-SRC in HG-HDFs stimulated with EA after 48 h. (G–I) Statistical analysis of (F). (J) Representative Western blot images of EGFR in HDFs and HG-HDFs. (K) Statistical analysis of (J). (L) Western blot analysis showing the expression levels of EGFR, INSR and C-SRC in HG-HEKs stimulated with EA after 48 h. (M–O) Statistical analysis of (L). (P) Representative Western blot images of EGFR in HEKs and HG-HEKs. (Q) Statistical analysis of (P). Data were analysed using one-way ANOVA with Tukey's post-test and presented as mean ± SD. #p < 0.05 ##p < 0.01,

Article Snippet: The membrane underwent blocking with QuickBlock Blocking Buffer (Solarbio, Beijing, China) for a duration of 15 min, after which it was incubated overnight at 4°C with primary antibodies including anti- CD63, anti- CD9, anti- TSG101, anti- Calnexin, anti- α- SMA, anti- COL1 (1:1000, Proteintech, Wuhan, China), anti- EGFR, anti- C- SRC (1:5000, Proteintech, Wuhan, China) and anti- INSR (1:2000, Proteintech, Wuhan, China).

Techniques: Western Blot, Expressing